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Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (Beyotime, China) at RT for 10 min

Using LC-MS/MS, we confirmed that the OspR modification successfully incorporated Orn into the semaglutide analogue through RiPPs

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RFM plus liraglutide, a GLP1 receptor agonist commonly prescribed for obesity and diabetes
