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After 24 h culture, the transfected cells were seeded into 384-well microtiter plates at a density of 3,000 cells per well in HBSS supplemented with 5 mM HEPES, 0.1% (w/v) BSA or 0.1% (w/v) casein and 0.5 mM 3-isobutyl-1-methylxanthine

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The purity tests (HPCL assay showing 99% purity, etc) are testing ONLY the purity of the peptide portion of the total mix (which is very tiny)

Additionally, neural and enteric reflex modulation by incretin agonism could alter mesenteric microvascular tone, although this mechanism remains speculative

Ghrelin is another possible player, since it has been proven to be expressed not only in the gut, but also in a distinct subpopulation of islet cells named -cells (231) and, being known to be a stress-induced (232) GLP-1 secretagogue (233, 234), it might play an important role in the intra-islet signaling