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The PCR protocol was as follows: initial denaturation (95C for 30 sec) followed by 40 cycles of denaturation (95C for 30 sec, 60C for 30 sec and 72C for 30 sec) and annealing and extension (72C for 1 min)

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This approach allows you to have direct access to specialists who can offer guidance and support whenever you need it

However, as indirect costs typically manifest over extended periods, they are excluded from this study, which adopts a short-term time horizon focusing on direct medical costs

Whilst NICE has not yet issued specific guidance on retatrutide reflecting its investigational status existing NICE and NHS frameworks for GLP-1 receptor agonists and nutritional deficiency management provide a relevant and applicable foundation for clinical practice