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DSY, DSC, and MHL performed the experiments

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By doing so, we aim to clarify how this coding system works, why it is important, and how it affects both healthcare providers and patients

Quantitative real-time PCR was performed in 20 l of reaction mixture containing 1 l of cDNA, 10 pM of each primer ( Slc1a5 , Slc1a5 variant Gls1 , Gls2 , Cdkn2a , Cdkn1a , Cd38 , Igf1r , Il-1b , Il-6 and Il-10 ) and 10 l of SYBR Select Master Mix (Thermo Fisher Scientific) using Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific)