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How to Decide: Discontinuation vs

Twenty-four hours after transfection, cells were lysed using 1 mL of TNTE lysis buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 5 mM EDTA, and 0.3% Triton X-100) supplemented with 1 PhosSTOP (Roche) and 1 EDTA-free Complete Protease Inhibitor Cocktail (Roche)

Knowing what to expect at each phase prevents two common mistakes: getting discouraged early when the scale moves slowly, and getting impatient mid-treatment when the rate of loss naturally slows

Each trial is enrolling 1,840 participants for 156 weeks of treatment

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