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Anesthesia induction and maintenance were carefully monitored, and the depth of anesthesia was adjusted to ensure the well-being of the animals throughout the experimental procedures

Single molecule fusions of GLP-1 to the peptide hormones gastrin, amylin, and cholecystokinin have been reported, as have fusions of GLP-1 to larger proteins such as FGF21, and to inhibiting antibodies against PCSK9

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After withdrawal of the needle, the area was swabbed to remove any excess tracer
