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Neurometabolic evidence supporting the hypothesis of increased incidence of type 3 diabetes mellitus in the 21st century

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Diabetologia 55, 24562468

For surface downregulation, labeling was performed with SNAP-Surface-488 in MIN6B1-SNAP-GLP-1R cells before fixation, or with anti-rodent GLP-1R (2 g l 1 ) in fixed MIN6B1 and INS-1 832/3 cells plus FITC-conjugated anti-mouse secondary (F0257, Sigma, 1/200)