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4 Semaglutide treatment in DIO-MASH and CDA-HFD mice: effects of treatment duration on liver fibrosis

Sjolund K, Sanden G, Hakanson R, Sundler F

sgRNAs against exons 4 and 5 of the Gipr , Glp-1r and Gcgr genes (ESM Table 2) were designed using CRISPOR tool (www.crispor.tefor.net), in vitro transcribed (E3322, NEB) and purified using the MEGAclear Kit

This conservative approach minimizes side effects while allowing gradual titration to your optimal therapeutic dose

However, the somewhat high prize of GLP1RAs poses barriers to treatment access, which may be an even greater challenge for patients with AUD compared to patients with diabetes and/or obesity