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In rectosigmoid biopsies from patients with constipation-predominant IBS and healthy controls, GLP-1R was significantly downregulated in IBS individuals compared to controls [55]

Real-Time PCR A total of 2 g/sample was subjected to cDNA synthesis using a cDNA reverse transcription kit (Cat # 4368814, Applied Biosystems, Waltham, MA, USA) according to the manufacturers protocol