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Competition binding with human GLP-1(7-36)NH 2 , GIP(1-42), tirzepatide, and semaglutide was performed essentially as described for homologous competition except that the assay buffer was 1.0 mM MgCl 2 , 2.5 mM CaCl 2 , 0.003% w/v Tween-20, 0.1% w/v bacitracin in 25 mM HEPES, pH 7.4, final concentrations with one Complete EDTA free protease inhibitor tablet added per 50 mL of buffer