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In a similar protocol, the in vitro and in vivo effects of metformin on mitochondria function were investigated in vitro in primary murine hepatocytes and Hepa 1-6 cells (murine hepatoma cells derived from the BW7756 tumor) and an in vivo protocol with high-fat diet (HFD) treated mice (138)

CD4+ CD25+ Tregs are a subpopulation of T cells that are effective in reducing inflammation (44, 45)

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