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The 20mg/ml concentration amplifies both risks because the margin of error is smaller
After washing three times, cells were resuspended in 200 L PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm, respectively
Adding a caloric deficit on top of existing marginal iron status can push levels into deficiency territory quickly
