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The conjugation of myristic acid results half-life extension from 4-6 min (insulin) to 5-7 hours (insulin detemir)

So a 5 mg vial contains 5,000 mcg, and a 250 mcg dose is 0.25 mg
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Three things do most of the work: lift weights several times a week, eat plenty of protein (I aim for about 0.7 to 1 gram per pound of goal body weight), and don't crash your calories too fast

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