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BioRad, Hercules, CA, USA) and real-time quantitative PCR (qPCR) was performed using a TaqMan 7900 Sequence Detection System with TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA)

This medication activates receptors for glucagon-like peptide-1 (GLP-1), a hormone released in the gut in response to eating that signals fullness and satiety


Compared with GLP-1 RAs, more participants receiving tirzepatide 15 mg discontinued treatment due to adverse events (OR 2.29 [95% CI 1.39, 3.75], I 2 0%), whereas no difference was evident for tirzepatide 5 mg and 10 mg (ESM Table 3)

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