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Terminal dUTP Nick End-labeling Staining Assay Assay Terminal dUTP nick end-labeling (TUNEL) staining assay was performed on H9c2 cells by using an in situ cell death detection kit (Roche Diagnostics) to evaluate cell apoptosis ( 5 cells/well) overnight before treatment with various agents in serum-free medium condition

These dysesthesia events were generally mild and rarely led to treatment discontinuation