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GLP-1R-SmBiT V229A was generated from the WT template by site-directed mutagenesis using the following primers: Forward (5 to 3): TCTTTCCTGTCGACTCGCGTTTCTGCTTATGCAGT, Reverse (5 to 3): ACTGCATAAGCAGAAACGCGAGTCGACAGGAAAG

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Then we build a comprehensive treatment plan

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