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Selkoe DJ

Immunohistochemical detection of alpha and beta cells was performed using rabbit anti-insulin (3014, 1:800, Cell Signaling Technology) and mouse anti glucagon (G2654, 1:1,000, Merck) as primary antibodies and goat anti-rabbit AF750 (A21039, 1:100, Invitrogen) and donkey anti-mouse AF555 (A32773, 1:200, Invitrogen) as secondary antibodies

Graaf C, Donnelly D, Wootten D, Lau J, Sexton PM, Miller LJ, et al

This is the conservative approach

Following propensity score matching, the final cohort comprised 95,190 GLP-1RA users and 95,190 DPP-4i users
