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Male ( n = 8) and female rats ( n = 8) were injected with i) semaglutide (0.026 mg/kg) and bupropion (10 mg/kg), ii) vehicle and semaglutide, iii) bupropion and vehicle, or iv) two vehicle injections at the three weekly drinking days (Monday, Wednesday, Friday)

2.5 Protein digestion and peptide labelling The flowchart of proteomics and bioinformatics analysis is shown in Figure 1

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