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After deep anaesthesia with isoflurane (Attane Vet, 1,000 mg g 1 , ScanVet, Piramal Critical Care) mice were decapitated and the brain was removed and submerged in ice-cold aCSF (124 mM NaCl, 5 mM KCl, 1.2 mM Na 2 HPO 4 2H 2 O, 2.7 mM CaCl 2 2H 2 O, 1.2 mM MgSO 4 (anhydrous)), 10 mM dextrose, 26 mM NaHCO 3 was adjusted to pH 7.4 and an osmolarity of 298302 mOsm kg 1 after saturation with carbogen (95% O 2 /5% CO 2 )

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Before you lost weight, your appetite was too big, Hill said

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[DOI] [PMC free article] [PubMed] [Google Scholar] 39.Hernn MA, Alonso A, Logan R, Grodstein F, Michels KB, Willett WC, et al

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Pituitary adenylyl cyclase-activating protein induces the voltage-independent activation of inward membrane currents and elevation of intracellular calcium in HIT-T15 insulinoma cells

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