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MIN-6 cells were transfected with constructs encoding the human FLAG-tagged GLP-1R in the hygromycin-resistant vector pcDNA3.1 with Lipofectamine 2000 (Thermo Fisher Scientific, Massachusetts, USA) 3,8,43

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Until more information is available, NAD+ Injection is only initiated in non-pregnant adults, and women of childbearing potential are typically asked about pregnancy status before starting therapy

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