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doi: 10.1056/NEJMoa2107519 37 FriasJPNauckMAVanJKutnerMECuiXBensonCet al

Cells were harvested by filtration through 500-m Nitex mesh and centrifuged twice at 1000 rpm for 10 min to eliminate broken cells and organelles

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62 Therefore, the existence of the cephalic phase of insulin secretion remains controversial and it appears to have minimal, if any, physiological relevance

Continuing or optimising metformin, adding basal insulin, or considering pioglitazone in specific circumstances are evidence-based alternatives that target different aspects of type 2 diabetes pathophysiology