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The washed membranes were homogenized in 30mM Hepes, pH 7.5, 150mM NaCl, 100uM TCEP, 2.5 mg/ml Leupeptin, 0.16 mg/ml Benzamidine

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Data and code to recreate figures in the study can be accessed at Change history 18 June 2024 A Correction to this paper has been published: References SAMHSA, Center for Behavioral Health Statistics & Quality

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Advanced / Aggressive Protocol (3 mL = ~10.0 mg/mL) Note: The 12 mg dose represents the highest dose tested in Phase 2 trials [3] [4] , producing maximum weight-loss efficacy (~24% body weight at 48 weeks)