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Brains and NG (when required) were extracted and post-fixed in 4% formaldehyde at 4C overnight (2 hours for NG), before being cryoprotected in 2030% sucrose solution for 24 hours at 4C
Sticking to a routine also minimizes the chances of side effects and lowers the risk of missing doses, which could slow your progress

Further corrections regarding the potentially non-isotropic GCGR expression could be attempted, but regardless the impact on the occupancy is expected to be low, while potentially introducing noise to the assessment
That means looking at your hormones, your nutrition, your stress levels, and your lifestyle not just writing a prescription and wishing you luck

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