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Next, we performed CLSM to examine the 24 h biofilms of nicotine-treated USA300 cells, using LIVE/DEAD staining, the viable cells of which were stained by green fluorescence (SYTO9) and dead cells by red fluorescence (PI)

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[41] [42] A range of extra-intestinal symptoms, said to be the only manifestation of NCGS in the absence of gastrointestinal symptoms, [22] [41] [42] have been suggested, but remain controversial

Pradeep Bhide, Ph.D., director of the Center for Brain Repair at the Florida State University College of Medicine, led a team of scientists who showed that the cognitive effects of nicotine use persisted through three generations of male mice