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2 L of RNA was used in a reaction containing a final concentration of 1x Reaction Mix with ROX, 300 nM of forward primer, 300 nM of reverse primer, 100 nM of probe and 0.4 L of SuperScript III RT/Platinum Taq Mix

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The Montecristo Epic 12 began with an abundant crop of Dominican and Nicaraguan tobacco that proved to be one of the finest harvests the countries had ever seen

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