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85 NMDA receptor channel Harmaline competitively displaces tritiated MK801 (dizocilpine) from the NMDA receptor in rabbit IO fractions (IC 50 60 M), 86 leading to the suggestion that harmaline induces tremor by acting as an NMDA receptor inverse agonist

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Fluorescein diacetate (FDA) was prepared as a stock solution in acetone at 1 mg/ml and immediately before use, was diluted 10-fold in wash buffer