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They evaluated the samples in a room-size environmental chamber at Berkeley Labs Air Quality Testing Laboratory, with additional tests carried out at the Molecular Foundry, a DOE Office of Science user facility at Berkeley Lab

Twenty-four healthy adult men who met Diagnostic and Statistical Manual of Mental Disorders, fourth edition (DSM-IV) criteria for nicotine dependence provided informed consent

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However the researchers had difficulties to evaluate which toxins were responsible for killing the fishes, due to the extremely high number of toxins that were found including nicotine, tar, arsenic and heavy metals like lead, copper and chrome