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After gel solidification, coverslips were removed and slides were immersed in freshly prepared ice-cold lysis solution (2.5 M NaCl, 100 mM Na 2 EDTA.H 2 0, 10 mM Tris HCL, NaOH, pH 10) with 10% Dimethyl Sulfoxide (DMSO, Sigma) and 1% Triton X-100 (Sigma), for 1 h, at 4C, in the dark

In these cases, professional guidance is essential to weigh risks against any potential benefits

and, they share four children

A high-throughput system for cyclic stretching of precision-cut lung slices during acute cigarette smoke extract exposure