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Investigations on the gonadal toxicity of Afzal, a common type of ST in Oman, found that oral administration of Afzal aqueous extracts to Wistar albino rats resulted in reduced levels of estrogen after the treatment period, and histological examinations revealed a decrease in germ cell number with deformed organization in ovaries (Al-Mukhaini et al., 2020)

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The mobile phase consisted of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B), and the flow rate was 0.3 mL/min

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