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They concluded ECV can elevate level of damaged mitochondrial DNA in circulating blood and induce the expression of toll-like receptor 9 (TLR9), thereby increasing the expression of proinflammatory cytokines in monocyte/macrophage, which is responsible for atherosclerosis

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They will then complete a series of self-administered forms (Drug Abuse Screening Test [DAST-10] [26], Depression, Anxiety and Stress Scale [DASS 21] [27], Fagerstrm Test for Nicotine Dependence [FTND] [28], Alcohol Use Disorders Identification Test [AUDIT] [29], 36-Item Short-Form Health Survey [SF-36] [30, 31], Questionnaire of Smoking Urges - Brief [QSU-brief] [32, 33], Patient Health Questionnaire [PHQ-9] [34], Minnesota Nicotine Withdrawal Scale [MNWS] [35], and modified Cigarette Evaluation Questionnaire [mCEQ] [36]) and staff-administered forms (demographic questions, history of smoking and quit attempts, social exposure to smoking, substance use, medical history and medical assessment, including vital signs, height and weight and concomitant medication record)

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