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Following the discovery that nicotine alters the expression of the PRM1 and PRM2 genes, further studies were conducted to investigate whether various nicotine products, such as smoking and hookah, had a different impact on the expression of these protamines [86,96,97,104,105,106]

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Adding a fungicide to a highly resistant variety may help reduce pathogen populations that may be able to colonize new host resistance traits
its more a process to ferment the tobacco
