first person to smoke tobacco History of How the first pipes appeared
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To our knowledge, current research in this area is limited

The most representative biomarkers are cotinine, a metabolite of nicotine, and t,t-muconic acid (TTMA), a metabolite of benzene, which are quite attributable to their specificity and sensitivity among the various urinary metabolites of tobacco 13,14

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Similar to cannabis, the sensitivity analysis for nicotine based on human data resulted in much lower MOE values