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BMJ 2019;366:l5275

They permit different levels of study: organ, tissue, cell (one or several populations), they allow large screening of effects with a very small amount of test material and are very well adapted for the study of mechanisms, mainly for short term studies [17, 20, 21]

Lastly, aqueous extracts are not suitable to ALI cell models since the application of these extracts is based on cell submersion, a non-physiological exposure method that annihilates the benefits of ALI

[41] One of the primary features of the more recent generation of devices is that they contain larger batteries and are capable of heating the liquid to a higher temperature, potentially releasing more nicotine, forming additional toxicants, and creating larger clouds of particulate matter