tobacco vasoconstriction Effects of Nicotine Contained in Mainstream Smoke on Vascular Smooth Muscle Cells 2 Overview of Pathophysiological Mechanisms
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His practical application of this observation resulted in the development of cross protection, a method that uses a mild strain of virus to protect plants from a subsequent infection by a more severe (economically important) strain of the same virus (22,24)

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Cell were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 IU/ml penicillin, and 100 g/ml streptomycin, and maintained at 37 C in a humidified atmosphere containing 5% CO 2

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