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*Correspondence: Hongwei Hou, [email protected] Hu, [email protected] These authors have contributed equally to this work and share first authorship This article was submitted to Neuropharmacology, a section of the journal Frontiers in Neuroscience Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

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If cotinine is present above this threshold, a positive result is triggered, successfully distinguishing active nicotine users from non-users