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5 Isolation and culturing strategies of tobacco microorganisms Traditional microbiological methods have played a crucial role in successfully isolating numerous microorganisms of interest and continue to be invaluable tools for cultivation (Figure 3)

John Gerard in his herbal of 1597, noted that tobacco was easy to cultivate and being now planted in the gardens of Europe, it prospereth very well and commeth from seede in one year to bear both flowers and seedeTobacco must be sown in the most fruitfull grounde that may be founde, carelessly cast abroade in the sowing, without taking it into the ground or any such pain or industrie taken, as is requisite in the sowing of other seedes. He also lists the names under which he knew the plant such as petun (native), sacra herbs or sancta herba (Lobel and Pena), hyoscyamus peruvianus or henbane of Peru , tabacum (Niccolaus Manardus) as well as nicotiana (Leonhart Fuchs)

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