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The evaluation of procollagen I synthesis, and IL-6 and TIMP-1 production were assessed by ELISA with the Human Pro-Collagen I alpha 1 ELISA kit (#ab210966, Abcam, Waltham, MA, USA), the Human IL-6 ELISA kit (#ab178013, Abcam, Waltham, MA, USA) and the Human TIMP1 ELISA kit (#ab187394, Abcam, Waltham, MA, USA), while MMP-1 activity was evaluated with the Human Active MMP-1 Fluorokine E Kit (#F1M00, R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions

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Discussion Despite considerable between-study variation in design, methods and populations, and many studies involving few subjects, the data, with few exceptions, show that HDL-C increases following quitting

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When these survival rates are compared to those of non-smoking patients, there is a 76% increase in the risk of premature mortality in persistent smokers, while this increase in risk decreases to 21% in those who quit smoking after angioplasty