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RT-qPCR The total RNA of cultured epithelial cell lines was extracted by using TRIzol (Invitrogen, CA, USA) reagent according to the manufacturers protocol

[234] Instead, he appeared in supporting roles in the disaster film Airport '77 (1977) with Jack Lemmon, the remake of The Big Sleep (1978) with Robert Mitchum as Philip Marlowe, and the family film The Magic of Lassie (1978)

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President Donald Trump gave his stamp of approval last week to raising the federal age requirement of who can legally purchase tobacco products to 21 when he signed spending bills approved by Congress this week