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Airliquid interface model for in vitro cell exposure treatment to aerosols In order to conduct exposure experiments with CS and ECS, the BEAS-2B cells were seeded onto transwell inserts with a 3 m polycarbonate membrane at a density of 2.0 10 4 cells/cm 2 , and an ALI was established by removing the medium from the apical surface, leaving the basal surface of the cells exposed to medium

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