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The most important difference between the 2011 and 2020 estimates for Spain is that smokers were included in the 2020 AM estimate

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This means that the system is particularly designed to be activated by aversive stimuli. To demonstrate this for nicotine, Liu and her colleagues infused the drug into mice and measured the second-by-second release of dopamine in the brain using a recently developed technique called dLight-based fiber photometry