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Protease was neutralized with FBS, the supernatant was saved (tube 1), and tracheal sections washed (5 mM HEPES, 5 mM EDTA, and 150 mM NaCl) for 20 min at 37 C

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Gwynn had to have surgery to remove a malignant growth in his mouth in 2010, and the word from Tom Friend of ESPN.com is that Gwynn had to have another surgery on Tuesday to remove a new cancerous tumor in the same spot where he had his last surgery