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The more we learn about this, the more we can handle things properly
Cells were trypsinized, pelleted, and washed with ice-cold phosphate-buffered saline (PBS) followed by resuspension in radioimmunoprecipitation assay (RIPA) buffer supplemented with 1X Protein Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) (Roche)
Itll change from pulsing to solid
Psychol Bull 143(10):10821115
This usually happens because air is trapped in the fuel tank
