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The effect of CSE treatment on epithelial cells was assessed with the human alveolar basal epithelial cell line A549, the human nasal epithelial cell line RPMI2650, the human bronchial epithelial cell line BEAS-2B, or the mouse lung epithelial cell line MLE12

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The ethics committee/institutional review board waived the requirement of written informed consent for participation from the participants or the participants legal guardians/next of kin because informed consent was waived by the IRB since only de-identified information was used

Balansky R, Ganchev G, Iltcheva M, Nikolov M, Steele VE, De Flora S (2012) Differential carcinogenicity of cigarette smoke in mice exposed either transplacentally, early in life or in adulthood