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[27] A 2015 review concluded that "Nicotine acts as a gateway drug on the brain, and this effect is likely to occur whether the exposure is from smoking tobacco, passive tobacco smoke or e-cigarettes." [28] Nicotine may have a profound impact on sleep

NF-B activation was quantified using a dual-reporter assay (QUANTI-Blue/QUANTI-Luc), and IL1B production was measured by ELISA to assess functional innate immune responses

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