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(2018) found a high frequency of saprophytic taxa among soil fungi affected by drought

RNA isolation and real-time PCR Total RNA from cells exposed to extracts for 48 h was extracted using TRIzol reagent (Invitrogen) and quantified using a nano-drop followed by Reverse Transcriptase PCR using random primers per manufacturers recommendations

Food products are classified into 4 groups according to their degree of processing: Unprocessed or minimally processed foods Processed culinary ingredients Processed foods Ultra-processed foods The determination of the group is based on the category of the product and on the ingredients it contains

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