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Through the interaction of these intermediates, exposure to tobacco smoke can directly or indirectly cause the formation of DNA and protein adducts, mutations, chromosomal abnormalities, micronucleus formation, sister chromatid exchange (SCE), and promoter methylation (Fig

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Moreover, by the end of the XIX century nicotines empirical formula was described (1843, Louis-Henri-Frederic Melsens), the structure was discovered (1893, Adolf Pinner) and in 1904 pure nicotine was first synthesized by a Swiss Ame Pictet