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MSCs cultured in osteogenic conditions displayed reduced expression of alkaline phosphatase mRNA, significantly reduced type I collagen (COL1) mRNA expression and a reduction in mineralisation following treatment with E-cigarette smoke extract [83]

The pH levels of smokeless tobacco products vary widely, from pH 5 to 8

However, the chronic accumulation of senescent cells can impair wound healing, accelerate aging, promote inflammation, and give rise to chronic diseases such as CVDs, cancer, and neurodegenerative diseases [9,10,11]

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Examples of non-animal alternative methods Organs-on-chips are tiny 3D chips created from human cells that look and function like miniature human organs
