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This addiction primarily stems from the presence of nicotine, a potent psychoactive substance found in tobacco, according to an article by the Center of Addiction and Mental Health titled Nicotine Dependence. The addictive nature of tobacco leads individuals to develop a tolerance, necessitating higher amounts to achieve the same effects, ultimately reinforcing the cycle of dependence

The strain was routinely recultured on potato dextrose agar (PDA: 200 g of potato, 20 g of glucose and 15 g of agar in 1000 mL water) at 27 in the dark for 15 days 59