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The e-liquid typically consists of a solvent (propylene glycol or vegetable glycerin), flavorings (eg, tobacco, mint, fruit, bubblegum), and, often, nicotine in various doses

Batch correction was performed using the Combat algorithm from the SVA package (68)

Nicotine and the Brain Nicotine stimulates release of dopamine from nucleus accumbens (brain reward center) Dopamine enhances feelings of reward and pleasure Nicotine increases serotonin levels, mostly in the amygdala (brain emotional response center) Serotonin can relieve depression and anxiety Nicotine and the Brain Initial experience with smoking causes nausea, dizziness, and/or coughing in some individuals Initial experience with smoking causes pleasurable experiences in others (e.g., head rush, buzz, dizziness)

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