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doi: 10.3760/cma.j.issn.1009-2587.2019.11.013 205 HuangBLiHJiangQLiYJiangZCaoHet al

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RNA was reverse transcribed into cDNA (50 ng RNA per 20 l RT reaction) using High Capacity RNA to cDNA kits (Life Technologies) according to the manufacturers instructions, and the expression of GSTP1 (Taqman probe ID Hs00168310_m1) and the loading control 18S ribosomal RNA (Hs03003631_g1) assessed by qRTPCR analysis, as previously described (Smith et al, 2012), where 20 l individual reaction mixes (per well) contained 10 l Taqman Universal PCR Master Mix (Life Technologies), 1 l gene-specific Taqman probe, 1 l cDNA and 8 l sterile water